描述
AmpliTaq? DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli. It is the most thoroughly charaerized enzyme available for the PCR process and its recombinant nature and purification method provide unparalleled purity and reproducibility, vial-to-vial, lot-to-lot. Features of this enzyme:
AmpliTaq? DNA Polymerase is the most thoroughly charaerized enzyme available for the PCR process, a testimony to its overall utility and efficacy
Its thermal aivity profile makes it good for PCR applications
It is QC-tested to guarantee reproducible results
Good for PCRThe thermal aivity profile of AmpliTaq? DNA Polymerase is good for PCR applications because its optimal aivity is in the same range at which stringent annealing of primers occurs (55°C–75°C). The enzyme's half-life is ~40 minutes at 95°C, providing thermostability that meets the requirements of most difficult PCR applications. AmpliTaq? DNA Polymerase is supplied with GeneAmp? 10X PCR Buffer I. It is also available with GeneAmp? 10X PCR Buffer II and MgCl
2 Solution.
Ultrapure optionAlso available is AmpliTaq? 360 DNA Polymerase for ior PCR results with the broadest range of templates. Compared to the original AmpliTaq? DNA Polymerase, AmpliTaq? 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating baerial DNA sequences from the enzyme preparation. This ultrapure enzyme reduces false positive results, amplifies low-level target sequences, and when combined with the proprietary AmpliTaq? 360 Buffer Kit, promotes the amplification of a variety of templates. Also included with this produ is a 360 GC Enhancer to help work through challenging GC-rich templates.
For Research Use Only. Not for use in diagnostic procedures.
描述
?AmpliTaq DNA聚合酶是一種分子量為94 kDa,,重組DNA聚合酶的一種改進(jìn)形式表達(dá)獲得的水生棲熱菌(Taq)在大腸桿菌DNA聚合酶基因。它是徹底的特點酶可用于聚合酶鏈反應(yīng)過程中,其重組的性質(zhì)和純化方法提供的純度和重現(xiàn)性,瓶到瓶,很多很多。
這種酶的特點:
?AmpliTaq DNA聚合酶是徹底的特點酶可用于PCR檢測,證明其整體的實用性和性
它的熱譜,使其的應(yīng)用程序
它是質(zhì)量控制測試,以可重復(fù)性的結(jié)果
和的聚合酶鏈反應(yīng)
?的AmpliTaq DNA聚合酶的熱譜是PCR應(yīng)用好因為它的活動是在引物退火時嚴(yán)格相同的范圍(55°C–75°C)。酶的半衰期是95°~ C 40分鐘,提供的熱穩(wěn)定性,滿足困難的PCR應(yīng)用的要求。?AmpliTaq DNA聚合酶提供?03倍PCR緩沖液I也可與?03倍PCR緩沖液和氯化鎂溶液。
規(guī)格
| Polymerase: | AmpliTaq? DNA Polymerase |
|---|---|
| Hot Start: | No |
| Fidelity (vs. Taq): | 1 X |
| Reaion Format: | Separate Components |
| Produ Overhang: | 3'-A |
| Reaion Speed: | Standard |
| Final Produ Size(s): | 5 kb or less |
| Produ Size: | 250 units |
| Exonuclease Aivity: | 5' - 3' |
| Shipping Condition: | Dry Ice |
內(nèi)容及儲存
Contains 250 units of AmpliTaq? DNA Polymerase and Buffer I. Store at –15 to –30°C.











